Featured Publications
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Processive RNA polymerization and promoter recognition in an RNA World. Cojocaru, R., & Unrau, P. J. (2021). Science, 371(6535), 1225–1232. https://doi.org/10.1126/science.abd9191
Live cell imaging of single RNA molecules with fluorogenic Mango II arrays. Cawte, A. D., Unrau, P. J., & Rueda, D. S. (2020). Nature Communications, 11(1), 1283.
Fluorogenic RNA Mango aptamers for imaging small non-coding RNAs in mammalian cells. Autour, A., C. Y. Jeng, S., D. Cawte, A., Abdolahzadeh, A., Galli, A., Panchapakesan, S. S. S., Rueda, D., Ryckelynck, M., & Unrau, P. J. (2018). Nature Communications, 9(1), 656.
Publications
2021 -
Processive RNA polymerization and promoter recognition in an RNA World. Cojocaru, R., & Unrau, P. J. (2021). Science, 371(6535), 1225–1232.
Microchip RT-PCR Detection of Nasopharyngeal SARS-CoV-2 Samples. Cojocaru, R., Yaseen, I., Unrau, P. J., Lowe, C. F., Ritchie, G., Romney, M. G., Sin, D. D., Gill, S., & Slyadnev, M. (2021). The Journal of Molecular Diagnostics, 0(0).
RNA Peach and Mango: Orthogonal two-color fluorogenic aptamers distinguish nearly identical ligands. Kong, K. Y. S., Jeng, S. C. Y., Rayyan, B., & Unrau, P. J. (2021). RNA, rna.078493.120.
2016 - 2020
RNA detection with high specificity and sensitivity using nested fluorogenic Mango NASBA. Abdolahzadeh, A., Dolgosheina, E. V., & Unrau, P. J. (2019). RNA, 25(12), 1806–1813.
Structure and functional reselection of the Mango-III fluorogenic RNA aptamer. Trachman, R. J., Autour, A., Jeng, S. C. Y., Abdolahzadeh, A., Andreoni, A., Cojocaru, R., Garipov, R., Dolgosheina, E. V., Knutson, J. R., Ryckelynck, M., Unrau, P. J., & Ferré-D’Amaré, A. R. (2019). Nature Chemical Biology, 15(5), 472–479.
Genome-wide discovery of somatic regulatory variants in diffuse large B-cell lymphoma. Arthur, S. E., Jiang, A., Grande, B. M., Alcaide, M., Cojocaru, R., Rushton, C. K., Mottok, A., Hilton, L. K., Lat, P. K., Zhao, E. Y., Culibrk, L., Ennishi, D., Jessa, S., Chong, L., Thomas, N., Pararajalingam, P., Meissner, B., Boyle, M., Davidson, J., … Morin, R. D. (2018). Nature Communications, 9(1), 4001.
Crystal Structures of the Mango-II RNA Aptamer Reveal Heterogeneous Fluorophore Binding and Guide Engineering of Variants with Improved Selectivity and Brightness. Trachman, R. J., Abdolahzadeh, A., Andreoni, A., Cojocaru, R., Knutson, J. R., Ryckelynck, M., Unrau, P. J., & Ferré-D’Amaré, A. R. (2018). Biochemistry, 57(26), 3544–3548.
Structural basis for high-affinity fluorophore binding and activation by RNA Mango. Trachman, R. J., Demeshkina, N. A., Lau, M. W. L., Panchapakesan, S. S. S., Jeng, S. C. Y., Unrau, P. J., & Ferré-D’Amaré, A. R. (2017). Nature Chemical Biology, 13(7), 807–813.
Fluorophore ligand binding and complex stabilization of the RNA Mango and RNA Spinach aptamers. Jeng, S. C. Y., Chan, H. H. Y., Booy, E. P., McKenna, S. A., & Unrau, P. J. (2016). RNA, 22(12), 1884–1892.
2011 - 2015
Small RNAs derived from tRNAs and rRNAs are highly enriched in exosomes from both old and new world Leishmania providing evidence for conserved exosomal RNA Packaging. Lambertz, U., Oviedo Ovando, M. E., Vasconcelos, E. J., Unrau, P. J., Myler, P. J., & Reiner, N. E. (2015). BMC Genomics, 16(1), 151.
In vitro characterization of 6S RNA release-defective mutants uncovers features of pRNA-dependent release from RNA polymerase in E. coli. Ovando, M. O., Shephard, L., & Unrau, P. J. (2014). RNA, 20(5), 670–680.
RNA Synthesis by in Vitro Selected Ribozymes for Recreating an RNA World. Martin, L. L., Unrau, P. J., & Müller, U. F. (2015). Life, 5(1), 247–268.
RNA Mango Aptamer-Fluorophore: A Bright, High-Affinity Complex for RNA Labeling and Tracking. Dolgosheina, E. V., Jeng, S. C. Y., Panchapakesan, S. S. S., Cojocaru, R., Chen, P. S. K., Wilson, P. D., Hawkins, N., Wiggins, P. A., & Unrau, P. J. (2014). ACS Chemical Biology, 9(10), 2412–2420.
E. coli 6S RNA release from RNA polymerase requires σ70 ejection by scrunching and is orchestrated by a conserved RNA hairpin. Panchapakesan, S. S. S., & Unrau, P. J. (2012). RNA, 18(12), 2251–2259.
2006 - 2010
Binding and release of the 6S transcriptional control RNA. Shephard, L., Dobson, N., & Unrau, P. J. (2010). RNA, 16(5), 885–892.
Closing the Circle: Replicating RNA with RNA. Cheng, L. K. L., & Unrau, P. J. (2010). Cold Spring Harbor Perspectives in Biology, 2(10), a002204.
Conifers have a unique small RNA silencing signature. Dolgosheina, E. V., Morin, R. D., Aksay, G., Sahinalp, S. C., Magrini, V., Mardis, E. R., Mattsson, J., & Unrau, P. J. (2008). RNA, 14(8), 1508–1515.
Comparative analysis of the small RNA transcriptomes of Pinus contorta and Oryza sativa. Morin, R. D., Aksay, G., Dolgosheina, E., Ebhardt, H. A., Magrini, V., Mardis, E. R., Sahinalp, S. C., & Unrau, P. J. (2008). Genome Research, 18(4), 571–584.
Selection of an improved RNA polymerase ribozyme with superior extension and fidelity. Zaher, H. S., & Unrau, P. J. (2007). RNA, 13(7), 1017–1026.
Extensive 3′ modification of plant small RNAs is modulated by helper component-proteinase expression. Ebhardt, H. A., Thi, E. P., Wang, M.-B., & Unrau, P. J. (2005). Proceedings of the National Academy of Sciences, 102(38), 13398–13403.
RNA-Catalyzed RNA Polymerization: Accurate and General RNA-Templated Primer Extension. Johnston, W. K., Unrau, P. J., Lawrence, M. S., Glasner, M. E., & Bartel, D. P. (2001). Science, 292(5520), 1319–1325.
Constructing an RNA world. Bartel, D. P., & Unrau, P. J. (1999). Trends in Cell Biology, 9(12), M9–M13.
RNA-catalysed nucleotide synthesis. Unrau, P. J., & Bartel, D. P. (1998). Nature, 395(6699), 260–263.
Accessing rare activities from random RNA sequences: the importance of the length of molecules in the starting pool. Sabetti, P. C., Unrau, P. J., & Bartel, D. P. (1997). Chemistry & Biology, 4(10), 767–774.